Fig. 2

Immunomodulation properties of BM-MSC expanded in different supplements. a Inhibitory effect BM-MSC on CFSE labeled PBMC. Cells were co-cultured at three different PBMC:BM-MSC ratios upon PBMC stimulation. Each bar represents mean ± SD of % inhibition of three independent experiments. Immunomodulation of BM-MSCs expanded in PL and PR-SRGF was comparable but significantly lower when compared with FBS. Three BM-MSC batches were analyzed by two-way ANOVA. b PBMC proliferation was assessed by CFSE dilution method on CD45+ cells. One representative case is shown