Fig. 5

The effect Ex-4 or ADSC-CM on migration, proliferation and angiogenic factor secretion in keratinocytes. a, d Migration assay. Plated HaCaT cells were wounded and the injury line was marked. a Cells were cultured with ADSC-CM and/or 10 nM Ex-4. d Cells were co-cultured with or without ADCSs in the upper chamber of a transwell plate with or without Ex-4 treatment. Migration patterns were observed under a phase contrast microscope and photographed (×200). b, e Proliferation assay. b HaCaT cells were plated on 96-well plates and incubated with or without ADSC-CM and/or 10 nM Ex-4. e HaCaT cells were plated on transwell plates and co-cultured with ADSCs (in the upper chamber) and/or 10 nM Ex-4. After 24 h, cell proliferation was determined with a cell counting Kit-8 assay. Results are expressed as a percentage of the control. *p < 0.05 vs CON. c Western blotting assay. HaCaT cells were incubated with or without ADSC-CM and/or 10 nM Ex-4. After 24 h, cells were harvested and western blotting assay for VEGF was carried out. The results were analyzed through ImageJ software for Windows. *p < 0.05 vs CON, ^# p < 0.05 vs ADSC + EX-4