Fig. 6
Expression and subcellular localization of PKM2 in muscle of DM patients: impact on pyruvate oxidation. a Tissue extracts (20 µg) derived from muscle biopsies of control (CTR) and dermatomyositis (DM) donors were fractionated on SDS-PAGE and blotted against anti-PKM1, anti-PKM2, anti-phospho PKM2Y105 and anti-GAPDH. Representative blots of two samples (1,2) per condition are shown. Histograms show the expression of the proteins in arbitrary units (a.u.) ±S.E.M. of five different muscle samples. *p < 0.05 when compared to controls by Student’s t test. b Representative confocal images of control (CTR) and dermatomyositis (DM) cryostat muscle sections incubated with anti-PKM2 (green) and DAPI (blue). Images were taken at 20× and 60×. c Same as in a but blotted with anti-PDH, anti-phospho PDH, anti-PDK1 and anti-GAPDH. Representative blots of two samples (1,2) per condition are shown. b Histograms show the expression of the proteins in arbitrary units (a.u.) ±S.E.M. of five different muscle samples. *p < 0.05 when compared to controls by Student’s t test