Fig. 2

Printing of RPPA. a The scheme of printing of RPPA processed for PKM2 is shown magnified for details. One nl samples were spotted in triplicate. Black boxed negative controls of BSA; Magenta boxed standard curves of HCT116 cells; Brown boxed positive controls of murine IgGs; Green boxed samples from control donors; Yellow boxed PM samples; Red boxed DM samples; Blue boxed sIBM samples. Below are shown representative RPPAs processed with other antibodies. b The plot illustrates the linear correlation that exists between the fluorescence intensity (arbitrary units, a.u.) and the amount of PKM2 in HCT116 cell lysates. Protein content in the biopsies was calculated according to the fluorescence intensity obtained in the standard curve of HCT116 cells. For other details see Additional file 1: Figure S1