Fig. 4

MWA plus OK-432 activated systemic tumor-specific immune responses. IFN-γ secreting cells were analyzed by ELISPOT. Mononuclear cells were prepared from spleen and cultured with 4T1 or CT26 cells for 48 h. a Representative ELISPOT images from each group. b the ELIPSOT count in each group. Column, mean; error bars, SEM. *P < 0.05; ***P < 0.001; NS not significant. Data were pooled from two independent experiments with eight mice per group