Table 3 Projection of attainable negative selection assay depletion performance
From: Advances in rare cell isolation: an optimization and evaluation study
Incubation | Ltotal | Depletion factor | Lfinal | Bead efficiency (cells/µL) |
|---|---|---|---|---|
First depletion | 2.5 × 107 | 20× | 1.25 × 106 | 3.4 × 105 |
Second depletion | 1.25 × 106 | 50× | 2.5 × 104 | 1.4 × 105 |
Total depletion | 2.5 × 107 | 1000× | 2.5 × 104 | 3.0 × 105 |