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Table 3 Projection of attainable negative selection assay depletion performance

From: Advances in rare cell isolation: an optimization and evaluation study

Incubation Ltotal Depletion factor Lfinal Bead efficiency (cells/µL)
First depletion 2.5 × 107 20× 1.25 × 106 3.4 × 105
Second depletion 1.25 × 106 50× 2.5 × 104 1.4 × 105
Total depletion 2.5 × 107 1000× 2.5 × 104 3.0 × 105