Fig. 3
From: Advances in rare cell isolation: an optimization and evaluation study
Flow cytometry of fibroblast spiking experiment. a Gated CD45PE stained fibroblast cells in red. b Plot of CD45PE stained fibroblast subpopulation gated by fluorescence mode. c Purified leukocytes solution containing 2.5 × 107 normal cells and 1 × 104 fibroblast cells before depletion as gated in a. The subpopulation in red comprises granulocytes. The population in green comprises monocytes. d Sample after depletion as gated in a. The red population comprises mainly fibroblasts. e Unspiked purified leukocyte suspension gated for all cell populations before depletion. The red population comprises granulocytes (35%), the green population comprises monocytes (10.9%), the blue population comprises lymphocytes (32.9%) and the magenta population comprises debris (13.5%). f Enriched leukocyte population. The red population comprises granulocytes (1.1%), the green population comprises monocytes (0.9%), the blue population comprises lymphocytes (26.6%) and the magenta population comprises debris (50.4%)