Fig. 6
From: LncRNA MALAT1 promotes development of mantle cell lymphoma by associating with EZH2
MALAT1 facilitated phosphorylation of EZH2 at T350, which up-regulated MALAT1-binding activity. a The effect of CDK inhibitors on EZH2 phosphorylation. Immunoblot analysis of pT350-EZH2, total EZH2 in Mino and Jeko-1 cells following 12 h treatment with roscovitine (25 μM). b, c Results from qRT-PCR of MALAT1 bound to EZH2 in Mino (b) and Jeko-1 (c) cells. Signals were normalized by the total amount of MALAT1 mRNA in each case. d Western blot results revealed the expression of both total EZH2 and pT350-EZH2 significantly decreased in Mino and Jeko-1cells transfected with si-MALAT1. Phosphorylation of EZH2 at Thr 350 was inhibited in MALAT1 knockdown cells, normalized to total EZH2