Fig. 6

Structural and functional characterisation of established IPCs. Results of immunocytochemical and colorimetric characterisation of generated insulin producing cells. Pluripotent cell lines with stably introduced PDX1-VP16 and NKX6.1 transgenes were differentiated towards insulin producing cells. Transgenes expression was switched on by doxycycline at the stage of IPC progenitors or maturation of insulin producing cells. Differentiated cells were immunophenotyped with antibodies against MafA, Pax6, SLC30A8, somatostatin, tyrosine hydroxylase (all except TH counterstained with DAPI), and stained with dithizone. The images represent selected clusters of positive cells in the population of differentiated cells. Scale bar 100 µm