Fig. 1

The outline of the differentiation procedure of iPSC into insulin producing cells. Schematic representation of the procedure used for differentiation of iPS cells to insulin producing cells. Arrows indicate time points of induction of transcription factors expression with doxycycline (DOX). The experimental framework consisted of four stages: culture of iPS cells, generation of definitive endoderm cells, formation of pancreatic progenitor cells and development of mature insulin producing cells. The diagram presents composition of the media used at each step