Fig. 3

High resolution Z-stack confocal imaging OCSCC was performed to determine if α-SMA+ myofibroblasts (green; panel B) in the TM co-localized with the MSC markers Gremlin-1 (red; panel C) and CD90 (blue; panel D). On merged images (panel D), co-localization of gremlin-1 with CD90 yields a magenta color as indicated by the cross-hairs and white arrow. These cells are positive for the MSC markers gremlin and CD90 but not α-SMA. The nuclei are stained grey (panel A).