Fig. 7
From: Factors affecting long-term efficacy of T regulatory cell-based therapy in type 1 diabetes
Tregs survival and dependency on IL2. a Survival of expanded Tregs was assessed in vitro after release from GMP laboratory for further 8 more days in various concentrations of IL2: 0.0, 10.0, 100.0 and 1000 UI/mL. Tregs were cultured either alone (upper panel) or co-cultured with CD4+ T effector cells (middle panel). The survival of CD4+ T effector cells cultured alone is presented for comparison (bottom panel). Results are presented as medians (min.–max.) of the percentages of 7-AAD+ dead cells. Significant differences in Kruskal–Wallis ANOVA between cultures are shown: (*) Tregs without IL2 vs. Tregs with added IL2; (#) Tregs without IL2 vs. cocultures of Tregs and Teffs without IL2; ($) cocultures of Tregs and Teffs without IL2 vs. cocultures of Tregs and Teffs with IL2; (&) Tregs without IL2 vs. Teffs without IL2. b In vivo serum concentrations of IL2 were measured during the follow-up. The values for untreated controls (NO Tregs), patients treated with a single infusion (single dose) and patients treated with two infusions (two doses) of Tregs are presented as medians (min.–max.); the open circles represent values from particular patients. The p values and short line represents significance of Wilcoxon test comparisons immediately after the 2nd Tregs infusion