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Table 1 Intra-assay (as an estimation of the repeatability) and inter-assay (as an estimation of the reproducibility) precisions of single-platform Muse™ Auto CD4/CD4% system for CD4 T cells measurement, expressed in absolute number and in percentage

From: Performance evaluation of the touchscreen-based Muse™ Auto CD4/CD4% single-platform system for CD4 T cell numeration in absolute number and in percentage using blood samples from children and adult patients living in the Central African Republic

Range CD4 T cells in absolute number CD4 T cells in percentage
Samples* (intrarun assay) Intra-assay CV**** (%) Samples** (interrun assay) Inter-assay CV (%) Samples* (intrarun assay) Intra-assay CV***** (%) Samples** (interrun assay) Inter-assay CV (%)
<200 cells/µl 161.5 ± 14.9 9.2 199.7 ± 20.8 10.4 19.0 ± 0.8 4.3 16.0 ± 1.8 11.0
200–350 cells/µl 266.1 ± 15.1 5.7 252.8 ± 33.5 13.2 16.6 ± 0.8 5.1 13.7 ± 1.8 13.7
>750 cells/µl 1856.9 ± 194.7 10.5 723.6 ± 56.6 7.8 28.3 ± 1.0 3.7 36.2 ± 3.2 9.0
  Mean intra-assay CV*** 8.5 Mean inter-assay CV*** 10.4 Mean intra-assay CV*** 4.4 Mean inter-assay CV*** 11.2
  1. * Mean ± standard deviation of results obtained by tenfold repeating the same day acquisition on Muse™ Auto CD4/CD4% system of the blood sample of each range of CD4 T cells
  2. ** Mean ± standard deviation of results obtained by tenfold repeating on 3 separate days acquisition on Muse™ Auto CD4/CD4% system of the blood sample of each range of CD4 T cells
  3. *** Precision is the coefficient of variance (CV) obtained by dividing the standard deviation (SD) of all the intrarun or interrun measurements by their mean (CV% = SD × 100/mean); the mean CV is the mean of the 3 CVs calculated at 3 different counts of blood CD4 T cells
  4. **** Intrarun CVs obtained with BD FACSCount (Becton–Dickinson, Franklin Lakes, NJ, USA) previously estimated in Dar es Salam (Tanzania) was 6.8% for CD4 T cell counts expressed in absolute number and 5.0% for CD4 T cell counts in percentage [41]
  5. ***** Interrun CVs obtained with BD FACSCount (Becton–Dickinson, Franklin Lakes, NJ, USA) estimated in Dar es Salam (Tanzania) were 8.0% for low CD4 T cell counts expressed in absolute number and 7.9% for low CD4 T cell counts in percentage; and 7.0% for high CD4 T cell counts expressed in absolute number and 5.6% for high CD4 T cell counts in percentage [41]