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Table 1 Intra-assay (as an estimation of the repeatability) and inter-assay (as an estimation of the reproducibility) precisions of single-platform Muse™ Auto CD4/CD4% system for CD4 T cells measurement, expressed in absolute number and in percentage

From: Performance evaluation of the touchscreen-based Muse™ Auto CD4/CD4% single-platform system for CD4 T cell numeration in absolute number and in percentage using blood samples from children and adult patients living in the Central African Republic

Range

CD4 T cells in absolute number

CD4 T cells in percentage

Samples* (intrarun assay)

Intra-assay CV**** (%)

Samples** (interrun assay)

Inter-assay CV (%)

Samples* (intrarun assay)

Intra-assay CV***** (%)

Samples** (interrun assay)

Inter-assay CV (%)

<200 cells/µl

161.5 ± 14.9

9.2

199.7 ± 20.8

10.4

19.0 ± 0.8

4.3

16.0 ± 1.8

11.0

200–350 cells/µl

266.1 ± 15.1

5.7

252.8 ± 33.5

13.2

16.6 ± 0.8

5.1

13.7 ± 1.8

13.7

>750 cells/µl

1856.9 ± 194.7

10.5

723.6 ± 56.6

7.8

28.3 ± 1.0

3.7

36.2 ± 3.2

9.0

 

Mean intra-assay CV***

8.5

Mean inter-assay CV***

10.4

Mean intra-assay CV***

4.4

Mean inter-assay CV***

11.2

  1. * Mean ± standard deviation of results obtained by tenfold repeating the same day acquisition on Muse™ Auto CD4/CD4% system of the blood sample of each range of CD4 T cells
  2. ** Mean ± standard deviation of results obtained by tenfold repeating on 3 separate days acquisition on Muse™ Auto CD4/CD4% system of the blood sample of each range of CD4 T cells
  3. *** Precision is the coefficient of variance (CV) obtained by dividing the standard deviation (SD) of all the intrarun or interrun measurements by their mean (CV% = SD × 100/mean); the mean CV is the mean of the 3 CVs calculated at 3 different counts of blood CD4 T cells
  4. **** Intrarun CVs obtained with BD FACSCount (Becton–Dickinson, Franklin Lakes, NJ, USA) previously estimated in Dar es Salam (Tanzania) was 6.8% for CD4 T cell counts expressed in absolute number and 5.0% for CD4 T cell counts in percentage [41]
  5. ***** Interrun CVs obtained with BD FACSCount (Becton–Dickinson, Franklin Lakes, NJ, USA) estimated in Dar es Salam (Tanzania) were 8.0% for low CD4 T cell counts expressed in absolute number and 7.9% for low CD4 T cell counts in percentage; and 7.0% for high CD4 T cell counts expressed in absolute number and 5.6% for high CD4 T cell counts in percentage [41]
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Journal of Translational Medicine

ISSN: 1479-5876

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