Fig. 5
From: An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant
Histological appearance of 2 week old spheroids. Shown are two representative examples of HE (a–d) and Alcian blue (e–h) stained cross sections of spheroids after 14 ± 1 day of culture. HE staining of donor #9 (a, b) and #10 (c, d), with magnifications of a and c being shown in b and d respectively. Alcian blue staining of donor #9 (e, f) and #10 (g, h), with magnifications of e and g being shown in f and h respectively. Alcian blue stained the GAGs present in the ECM blue, whereas nuclear fast red stained the nuclei of the cells red. The samples were stained in different staining batches, causing a difference in observed staining intensity. Scale bar 100 µm