Fig. 2

r-Ang-2 suppresses inflammatory cell infiltration. a Recruitment of macrophages in response to ischaemia as determined by Mac-3 staining. Representative images of macrophages surrounding collateral arterioles in ischaemic adductor muscles in r-Ang-2 treated-mice on day 7 after femoral artery ligation are shown. b mRNA expression of CD11c (M1 marker), and c mRNA expression of CD206 (M2 marker) in ischemic adductor muscles as measured by real-time PCR. d Ratio of CD11c to CD206, The data were normalized to the expression level of 18S rRNA in each sample. All values represent the mean ± SEM (n = 6/group). *P < 0.05 vs. vehicle control. e Thioglycollate-elicited mouse peritoneal macrophages were used to evaluate macrophage migration. Cells were added to the upper chambers of cell culture inserts and incubated with Ang-2 (200 ng/mL) or vehicle control and then treated with MCP-1 (100 ng/mL) or vehicle control as indicated. Representative images of macrophages that migrated to lower-chamber side of transwell membrane. f The data represent the mean of triplicate experiments. *P < 0.05 vs. negative control (cells exposed neither MCP-1 nor Ang-2). **P < 0.05 vs. macrophages incubated with MCP-1 only