Fig. 4

Frequencies of HAdV-specific T cells in healthy donors and HAdV-infected HSCT recipients. Percentage of HAdV-specific CD3⁺CD8⁺ T cells as detected by pentamer staining. a PBMCs from healthy donors were analyzed for A01Hexon_TDLG-, A02Hexon_TLLY- and A01Penton_STDV-specific CD3⁺CD8⁺ T cells before (day 0) and after stimulation with the peptides A01Hexon_TDLG, A02Hexon_TLLY or A01Penton_STDV, respectively (day 7). Frequencies of A01Hexon_TDLG-, A02Hexon_TLLY- and A01Penton_STDV-specific CD8⁺ T cells, as detected by pentamer staining, are expressed as mean frequencies of HAdV-specific CD3⁺CD8⁺ T cells. b T-cell responses were assessed in HAdV-infected patients. Highest frequencies of A01Hexon_TDLG, A02Hexon_TLLY-, and A01Penton_STDV-specific CD8⁺ T cells as detected by pentamer staining, were shown for each patient and are expressed as mean frequencies of HAdV-specific CD3⁺CD8⁺ T cells. CD8⁺ T cells were stained with PE-labeled pentamers. The results of independent experiments are expressed as mean HAdV-specific CD3⁺CD8⁺ T-cell frequencies. Asterisks indicate statistically significant differences between the HAdV peptides (*p < 0.05, **p < 0.01, ***p < 0.001)