Fig. 1

Schematic overview of the experimental approach for the identification and evaluation of novel CD8⁺ T-cell epitopes. The identification and evaluation of epitope candidates were the two major steps of the general workflow. First, epitope candidates were mapped by reverse immunology using different prediction algorithms for peptide binding affinity and stability. Second, the highest-scoring peptide candidates were synthesized and evaluated for immunogenicity by T-cell pre-screening and T-cell immunoassay