Fig. 3

Representative images of non-PAD and PAD gastrocnemius muscle sections stained for mitochondrial activity and mitochondrial electron transport proteins. Top row shows succinate dehydrogenase (SDH) activity (dark fibers are type I, intermediate fibers are type IIa, and light fibers are type IIa/x), second row shows mitochondrial complex I, subunit 20 (orange), third row shows mitochondrial complex IV, subunit I (COX-1; green) staining and bottom row shows complex I and COX-1 merged. Arrows within the same column point to same fibers. Scale bar = 50 µm