Fig. 2

Representative images of serial PAD gastrocnemius muscle sections stained for mitochondrial activity and fiber type. A succinate dehydrogenase (SDH) activity; B myosin heavy chain (MyHC) composition by immunohistochemistry; C cytochrome c oxidase activity. Arrows point to the same fiber in serial sections. Arrow, type I fiber (pink); double arrow, type IIa fiber (green); large arrowhead, type IIa/x hybrid fiber (yellow/orange). D Correlation between the percent of total fibers with SDH cavities and type I fiber frequency in PAD subjects (n = 18, approximately 1000 fibers counted per subject). Scale bar = 100 µm