Fig. 1

Fiber type analysis of gastrocnemius muscle sections using isoform-specific myosin heavy chain (MyHC) immunohistochemistry. A Quantification (mean ± SEM) of type I (pink), IIa (green), and IIa/x (yellow/orange) muscle fibers, shown in representative images, of non-PAD (B; n = 7) and PAD (C–E; n = 26) participants. C–E) Representative images of PAD subjects with primarily type I fibers, 50 % type I and 50 % Type II, and primarily type II fibers, respectively; F individual variation in fiber type distribution between non-PAD and PAD subjects (approximately 1000 fibers analyzed per subject). Scale bar = 100 µm