Fig. 1

Experimental schedule of short-, intermediate- and long-term MPA/CsA incubation. CIK cells were generated by stimulation with interferon (IFN)-γ, OKT3, IL-2 and IL-15 over a period of 10 days. Subsequently, cells were split and either supplemented with 50 ng/ml IL-15 every 3 days (control) or additionally treated with 10 µM MPA or 5 µg/ml CsA. Proliferation, cytokine secretion and receptor expression were investigated following short- (24 h), intermediate- (3 days) and long-term (7 days) MPA treatment