Fig. 4
Effect of MFD on the liver fibrosis-related protein expression in CCl4-treated mouse liver. Histological examination of liver were revealed as indicated by immunohistochemical staining of TUNEL. Hydroxy-proline, α-SMA and COL-1 were evaluated by immunohistochemical staining. Representative liver sections stained as oil infusion control group (I); Mice with MFD treatment (II); Mice with CCl4 injection (III); MFD treatment (0.1 and 0.5 mg/kg) (IV, V); Magnification, ×200. The cells were counted from 10 fields (×200 magnification) of each liver sample. The results from statistical analysis are the means of cells and were calculated per microscope field from eight animals per group. Data are expressed as mean ± SD of independent experiments. *P < 0.05, as compared to the CCl4-treated group