Fig. 2

Effect of MFD on the expression of activated HSC-T6 markers and fibrosis-related proteins. HSC-T6 cells were treated with various concentrations of MFD for 48 h; then total cell lysates were tested for (a) TGF-β1, CTGF, α-SMA, COL-1 and p-p65 NFκB by western blot analysis. b HSC-T6 cells were treated with TGF-β1 (2 ng/mL) alone or in combination with different concentrations of MFD for 48 h; then total cell lysates were analyzed by western blot analysis against anti-CTGF, anti-α-SMA, anti-COL-1, and anti-β-actin as an internal control. Quantification of band intensity relative to α-tubulin is shown below the western blot. The quantitative data were presented as the mean of three repeats from one independent experiment. The data were presented as mean ± SD of three independent experiments. ^# P < 0.05 versus control. *P < 0.05, versus TGF-β1-treated alone