Fig. 4

Immunohistochemical staining on 3 months post-transplant kidney samples: Immune cell infiltration. Pigs were euthanized after 3 months follow up and kidney biopsies were collected and processed for immunohistochemical analysis. a MAC-387 staining, detecting macrophages in the pig. Left and middle representative staining for each group. Arrows designate positive cells. Magnification: ×100. Right quantification of the number of positive cells per high powered field (×100). b CD3 staining, specific for T lymphocytes. Left and middle representative staining for each group. Arrows designate positive cells. Magnification: ×100. Right quantification of the number of positive cells per high powered field (×100). Shown are mean ± SD, n = 7. Statistics: *p < 0.05. MAC-387 data were normally distributed but did not show equality of variance on the Variance-Ratio Equal-Variance Test, hence non parametric analysis was performed. CD3 data was normally distributed and had equal variance, hence parametric analysis was performed