Fig. 3

AMPKα activation in L6 myotubes. Cells were stimulated with either 100 nmol/l EXE, 100 nmol/l INS and both. After stimulation, whole lysates were immunoblotted with the phospho-specific antibodies: p-Akt S473 (a), p-GSK3β S21/9 (b), p-ERK1/2 T202/Y204 (c) and p-AMPK T172 (d). In order to normalize for protein levels, blots were stripped and re-probed with anti-AKT, anti ERK1/2, anti-GSK3β and anti-AMPK antibodies. Data are shown as mean ± SD of three independent experiments. (*p < 0.001 vs basal)