Fig. 2

Representative in vivo images of WT and CF mice transiently transgenized with bIL-8-Luc and intratracheally treated with Pseudomonas LPS (6.25 µg/mouse). a, d: WT and CF mice treated with EMEM (control). b, e: WT and CF mice after intratracheal instillation with LPS and monitored IL-8 activation at 4 and 24 h by BLI. c, f: ex vivo images of IL-8 activation in lungs excised from WT and CF mice at 24 hs after LPS challenge. Panel G: Time-course of IL-8-luc activation in WT and CF mice. Results are reported as folds of increase (FOI) over baseline as mean ± SEM, n = 6 at each group. h: Cellular infiltration into the lung of WT and CF mice after intratracheal instillation with LPS at 24 h. Three mice were used for every time point and the experiment was replicated three times. Values are expressed as mean ± SEM of the three different experiments. Statistical differences were tested by one-way ANOVA followed by Dunnett’s t post hoc test for group comparisons. *p < 0.05 and **p < 0.01 WT and CF TSB vs WT and CF LPS group. (I–n): Representative photomicrographs of Giemsa stained lung of WT and CF mice after intratracheal instillation with EMEM (I, L) and LPS (M, N) at 24 h. Magnification 40×