Fig. 1

Example of the SVF flow cytometric analysis. a CD16 positive monocytes were first identified and delineated in the blood sample (left, CD16positive macrophages in the upper part). The setting was fixed and subsequently used for the SVF analysis (b). Total macrophages in the SVF were identified by positivity for CD14 (c). Based on the CD16 marker, two subpopulations were distinguished (b, CD16-positive macrophages in the upper part). d The CD16+ subpopulation was divided according to CD36 marker markers (d, left) and then the CD163 presence was determined within both the CD36 positive subpopulations [i.e., CD16+ CD36^high (d, middle) and CD16+ CD36^low (d, right)]. Similarly, the CD16 negative subpopulation was divided according to the CD36 marker (e, left) and then the CD163 presence was determined in both the CD16− CD36^low (e, middle) and CD16− CD36− subpopulations (e, right)