Detection of tumor-associated cells in cryopreserved peripheral blood mononuclear cell samples for retrospective analysis

Table 1 Recovery efficiency of spiked tumor cell lines in cryopreserved human PBMC samples

Cell line	Input cells	Day 0^a		Day 7^b		Day 66		Mean (SD) %
Cell line	Input cells	Cells recovered, no.	Recovery, %	Cells recovered, no.	Recovery, %	Cells recovered, no.	Recovery, %	Mean (SD) %
MCF-7	4000	3620	90.5	3510	87.8	3560	89.0	89.1 (1.4)
PC-3	3000	2500	83.3	2560	85.3	2540	84.7	84.4 (1.0)
786-O	3000	2780	92.7	2480	82.7	2440	81.3	85.6 (6.2)

ISSN: 1479-5876