Table 3 List of primers used for quantitative qRT-PCR analysis
From: Immune response and innervation signatures in aseptic hip implant loosening
Gene | GenBank number | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|---|
B2M | [NM_004048] | CCAGCGTACTCCAAAGATTCAG | AGTCAACTTCAATGTCGGATGG |
β-actin | [NM_001101] | TACCTCATGAAGATCCTCA | TTCGTGGATGCCACAGGAC |
IL-1β | [NM_000576] | CTTCAGCCAATCTTCATT | CACTGTAATAAGCCATCAT |
IL-6 | [NM_000600] | CAATCTGGATTCAATGAGGAGACT | CTGTTCTGGAGGTACTCTAGGTAT |
IL-10 | [NM_000572] | GGAGAACCTGAAGACCCTCA | TATAGAGTCGCCACCCTGAT |
IL-12a | [NM_000882] | TACCAGGTGGAGTTCAAG | GTTCTTCAAGGGAGGATTT |
iNOS | [NM_000625] | AATTGAATGAGGAGCAGGTC | TCCTTCTTCGCCTCGTAA |
TNF-α | [NM_000594] | TCTCTCTAATCAGCCCTCTG | TGCTACAACATGGGCTACAG |
RANKL | [NM_003701] | GGATGGCTCATGGTTAGA | CAAGAGGACAGACTCACTT |
TGF-β1 | [NM_000660] | CCTGGACACCAACTATTG | CTTGCGGAAGTCAATGTA |