Fig. 4

Frequencies of Th22 and Tc22 cells in HCs and patients with LP. PBMCs obtained from patients with LP (n = 10, black circles) and HCs (n = 19, white circles) were left unstimulated or were stimulated with SEB for 16 h and assessed by flow cytometry. A representative gating strategy for the selection of CD4⁺ T cells is shown; the same strategy was used to select CD8⁺ T cells (a). Production of CD3⁺CD4⁺ and CD3⁺CD8⁺ (IFN-γ⁻IL-17⁻) T cells at basal levels (b) and following stimulation with SEB (subtracted from baseline levels) (c). The results are shown as the mean ± SEM. *P < 0.05, **P < 0.01 when compared with the HC group