Fig. 3

Optimization of EVs isolation from uterine aspirates for RNA analysis. a Schematic representation of the four conditions tested to isolate EVs from uterine aspirates in order to purify their RNA content. Modifications were introduced to the standard protocol of ultracentrifugation. Protocol “A”: Sonication prior to RNase A treatment was applied to isolated EVs. Protocol “B”: Sonication of isolated EVs. Protocol “C”: RNase A treatment of isolated EVs. Protocol “D”: No modifications were included. b Concentration and quality of RNA derived from each condition was analyzed with an Agilent Bioanalyzer. c Ct values of miR-200b, miR-200c, miR-223, miR-205, miR-17 and miR-106a are plotted for each Protocol