Fig. 1

Illustration of IFNγ/IL2 FluoroSpot assay images. Individual images were captured for IFNγ (left image) (FITC filter) and IL2 (central image) (PE filter) and used to generate the computerized overlay of the two filters showing double positive IFNγ + IL2 cell responses (right image). IFNγ, IL2 and IFNγ + IL2 secreting cells upon stimulation with BZLF1 are depicted as green (left image), red (central image), and yellow (right image) spots, respectively