Fig. 6

a Immunofluorescence analysis was performed for AdipoR1 and AdipoR2 in the HGECs with or without resveratrol treatment (original magnification, ×400). Effect of resveratrol on intracellular signaling in HGECs cultured in low glucose (LG, 5 mmol/L of d-glucose) or high glucose (HG, 30 mmol/L of d-glucose) with or without resveratrol (50 μM). AdipoR1 and AdipoR2, phospho-Thr¹⁷² AMPK, total AMPK, phospho-Ser²⁵⁶ FoxO1, total FoxO1, phospho-Ser²⁵³ FoxO3a and total FoxO3a were assessed in HGECs. Protein lysates (10 μg) were separated by SDS–PAGE and analyzed by western blot. b Representative results for AdipoR1 and AdipoR2, phospho-Thr¹⁷² AMPK, total AMPK, phospho-Ser²⁵⁶ FoxO1 and total FoxO1, phospho-Ser²⁵³ FoxO3 and total FoxO3a and β-actin. c Quantitative analyses for AdipoR1/β-actin, AdipoR2/β-actin and phospho-Thr¹⁷² AMPK, phospho-Ser²⁵⁶ FoxO1 and phospho-Ser²⁵³ FoxO3a, all of which are relative to totals. *p < 0.05 and **p < 0.01 compared with LG, LG + Res, and HG + Res. HGECs human glomerular endothelial cells, AdipoR adiponectin receptor, AMPK 5′-adenosine monophosphate-activated protein kinase, FoxO class O forkhead box, LG low glucose, Res resveratrol, HG high glucose