Fig. 5

Synovial macrophages in ApoE^−/− mice have an MHCII⁺ phenotype and exhibit characteristics of foamy macrophages. Flow cytometric analysis of synovium from 18-week-old non-arthritic and arthritic C57BL/6 (control) and ApoE^−/− mice (n = 4–5 mice/group). a Total number of synovial macrophages. b The ratio of MHCII⁺ to MHC II⁻ synovial macrophages. c Histogram of oxidized LDL scavenger receptor, CD36, is shown with concatenation of samples from one experiment of non-arthritic control (black) and ApoE^−/− (red) mice. Geometric mean illustrates CD36 expression in d MHCII⁻ and e MHCII⁺ macrophages of individual mice. f Histogram representing uptake of lipophilic dye (BoDIPY) in a concatenation of samples from one experiment of non-arthritic control (black) and ApoE^−/− (red) mice. No dye control (grey) is included. Cumulative data displayed as geometric means in g MHCII⁻ and h MHCII⁺ macrophages. i Histogram of side scatter (SSC) of concatenation of samples from one experiment of non-arthritic control (black) and ApoE^−/− (red) mice is shown with a composite of samples illustrated as geometric mean of j MHCII⁻ and k MHCII⁺ macrophages. Asterisk denotes statistically significant differences: *p < 0.05, **p < 0.01