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Table 2 Assay variability in saliva samples. (A) Variability in 1,5-AG measurements with GlycoMark assay. (B) Variability in osmolality measurements with FISKO osmometer

From: Measurement of 1,5-anhydroglucitol in blood and saliva: from non-targeted metabolomics to biochemical assay

 

Within-assay variability

Between-assay variability

Master mix

Master mix

Control low

Control high

(A) GlycoMark assaya

 Number of samples

20

59

42

42

 Average 1,5-AG, µg/mL

4.08

4.11

4.94

14.6

 CV,  %

1.70

2.05

1.80

1.38

(B) Osmolality measurementsb

 Number of samples

20

59

 Average osmolality, mOsmol/kg

59

59

 CV, %

1.31

1.40

  1. aTable shows that the GlycoMark assays yield reliable and reproductive measurements in saliva. The coefficient of variation (CV) is expressed in percentage and the assay read-out in µg/mL
  2. bTable shows that the osmolality measurements have a very low variability. The coefficient of variation (CV) is expressed in percentage and the osmolality in mOsmol/kg