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Table 2 Assay variability in saliva samples. (A) Variability in 1,5-AG measurements with GlycoMark assay. (B) Variability in osmolality measurements with FISKO osmometer

From: Measurement of 1,5-anhydroglucitol in blood and saliva: from non-targeted metabolomics to biochemical assay

  Within-assay variability Between-assay variability
Master mix Master mix Control low Control high
(A) GlycoMark assaya
 Number of samples 20 59 42 42
 Average 1,5-AG, µg/mL 4.08 4.11 4.94 14.6
 CV,  % 1.70 2.05 1.80 1.38
(B) Osmolality measurementsb
 Number of samples 20 59
 Average osmolality, mOsmol/kg 59 59
 CV, % 1.31 1.40
  1. aTable shows that the GlycoMark assays yield reliable and reproductive measurements in saliva. The coefficient of variation (CV) is expressed in percentage and the assay read-out in µg/mL
  2. bTable shows that the osmolality measurements have a very low variability. The coefficient of variation (CV) is expressed in percentage and the osmolality in mOsmol/kg