Fig. 3

MSC immunosuppressive properties. Inhibition of PBMC proliferation by third party MSC: PBMC (100,000 or 50,000) were stimulated (S-PBMC) with anti-αCD3/CD28 microbeads during 4 days with or without irradiated (25 Gy) MSC (10/1 or 5/1 PBMC/MSC ratios) added at the beginning of the culture. Proliferation was assessed by analysis of the cell cycle by flow cytometry (N = 28). Result are expressed as the percentage of cells present in S + G2 M phases (a) and as the percentage of inhibition compared to the stimulated PBMC condition alone (b) for the 10/1 PBMC/MSC ratio