Fig. 3

Down-regulated RACK1 attenuates cells proliferation. a After NPC cells transfected with RACK1 siRNA or si-control, the expression of RACK1 was detected by western blot. b MTT assay was applied to measure the cell growth. Two-way ANOVA test. c, d Beside, EdU assay was used to test the proliferation of NPC cells. Original magnification, ×100; scale bar 100 μm. Student’s t-test. e, f Plate clone formation assay also showed the difference of NPC cells proliferation. The data were shown as the mean ± sem Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001