Fig. 5

GIFT4 treatment expanded human T cells in NSG mice. PBMC from the CLL patients were adoptively transferred into NSG immune deficient mice, and treated with GIFT4, GM-CSF and IL-4 or PBS for 6 days. a On Day 7, peripheral blood were collected from the treated mice, and circulating human T cells in the blood were analyzed by FACS with anti-human CD3 antibody. T cell number per 100 μl blood was calculated based on bead counting. b On day 30, circulating human T cells in PBMC of treated mice were also profiled by FACS with anti-human CD3 antibody, and percentage of human T cells was presented (c). Data were from three independent experiments using samples from subjects No. 1, 3 and 7