Skip to main content
Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Metronomic temozolomide as second line treatment for metastatic poorly differentiated pancreatic neuroendocrine carcinoma

Fig. 3

Detection of MGMT methylation by MSP and MethyLight qMSP. Isolated DNA from tumor specimen is treated with sodium bisulfite, which changes unmethylated cytosine (C) into uracil (U), but not methylated cytosine (mC), which remains unchanged. The modified DNA is used as a template for MSP or MethyLight qMSP. (MSP/agarose gel electrophoresis) When MGMT in neuroendocrine tumor is methylated (DNA M), the band is high, while the unmethylated band (DNA U) is low. The gel shows the presence of methylation band in BT1, brain tumor a with strong methylated, DNA M, used as positive control, and unmethylation band in BT2, brain tumor a with strong unmethylated, DNA U, used as negative control

Back to article page

Journal of Translational Medicine

ISSN: 1479-5876

Contact us