Fig. 1

FCM analysis for MD-2/TLR4 complex. A Representative FCM diagrams of MD-2/TLR4 complex detection. SKOV3 cells were treated with AO-1(100 μmol/L) alone or combined with LPS (1 μg/mL) respectively. Next, MD-2/TLR4 complex was detected with FCM as described under “Methods” section. B Inhibition of AO-1 on LPS-induced MD-2/TLR4 complex in SKOV3 cells. All experiments were performed in triplicate, and data are expressed as mean ± SD (n = 3). Error bars represent SD of replicate data points. *P < 0.05, compared to the control