Fig. 2

Deregulation of antioxidant genes expression in the atria of WT and Nrf2^−/− mice in response to HIES. Quantitative RT-PCR determination of RNA message for Nrf2/ARE-regulated antioxidant genes in the atria of SED and HIES (WT and Nrf2^−/−, n = 4–6/group) mice. The respective Ct values were normalized to the Gapdh expression and the relative differences in target gene level were determined using the arithmetic formula 2^−ΔΔCt. The results are presented as mean ± SEM of WT-sedentary. *P < 0.05 vs SED WT; ^#P < 0.05 vs SED Nrf2^–/– and ^$P < 0.05 vs HIES WT