Fig. 2

Time course of p38, JNK and ERK MAPK pathway activation by IL-17A. NP cells were cultured in serum-free DMEM-F12 medium for 12 h to synchronize the cells and to make them quiescent before IL-17A stimulation. Immunoblot analysis of total cell extracts isolated from primary NP cells treated with 100 ng/ml IL-17A for various time. Equivalent amount protein (10 μg) were resolved on 10 % SDS-PAGE, electroblotted and immunoblotted using antibodies against (a, d) the phosphorylated forms of p38 (upper panel), or total p38 (lower panel); or (b, e) the phosphorylated forms of JNK (upper panel), or total JNK (lower panel); or (c, f) the phosphorylated forms of ERK (upper panel), or total ERK (lower panel). IL-17A treatment could induce phosphorylation of p38 and JNK, but no obvious phosphorylation of ERK