Fig. 3

PRL-3 inhibitor I induced growth arrest and inhibited cell survival at high concentrations in prostate cancer cells. PC3 (a + c) and DU145 (b + d) cells were seeded in quintuplets in a 96-well plate, incubated in media containing 10 % FCS and with PRL-3-inhibitor I or corresponding DMSO concentrations as indicated for 72 h. Cell growth was measured with CellTiter-Glo Luminescent Cell Viability Assay (a + b) or [3H]-thymidine incorporation (c + d). Relative luciferase units (RLU) reflect the amount of ATP detected in each well. Figure showing one representative out of four experiments. Error bars represent ± one SD of quintuple measurements. Cells were incubated with media containing 10 % FCS and with various PRL-3-inhibitor I or highest DMSO concentrations for 72 h. Survival of human prostate cancer cell lines PC3 (e) and DU145 (f) was determined by annexin V-FITC/propidium iodide flow cytometry. Figure showing one representative of three experiments. Error bars represent ± one SD of duplicate measurements