Table 2 Primer pairs used for RT-PCR and RT-qPCR studies
Gene ID | Accession number | Oligonucleotides (5′ →3′) | Amplicon size (bp) |
|---|---|---|---|
BMP7 | NM 001719 | FWD: TACGCCGCCTACTACTGTGA REV: CCGGACCACCATGTTTCTGTA | 219 |
CD45 | NM 002838 | FWD: AGCACCTACCCTGCTCAGAA REV: TTCAGCCTGTTCCTTTGCTT | 159 |
5′-EpCAM | NM 002354 | FWD: CAGGTCCTCGCGTTCGGG REV: CAGTCAGGATCATAAAGCCCATCA | 284 |
Middle EpCAM | NM 002354 | FWD: AATGGACCTGACAGTAAATGG REV: ATCTCAGCCTTCTCATAC TT | 216 |
3′-EpCAM | NM 002354 | FWD: TGGGGAACAACTGGATCTGG REV: GTTCCCTATGCATCTCACCCA | 227 |
5′-GAPDH | NM 002046 | FWD: GGAAGGTGAAGGTCGGAGTC REV: CTCGCTCCTGGAAGATGGTG | 237 |
Middle GAPDH | NM 002046 | FWD: GAGAAGGCTGGGGCTCATTT REV: AGTGATGGCATGGACTGTGG | 231 |
3′-GAPDH | NM 002046 | FWD: AAGGTCATCCCTGAGCTGAA REV: TGACAAAGTGGTCGTTGAGG | 271 |
IL6 | NM 000600 | FWD: AATGAGGAGACTTGCCTGGTG REV: GCTGCGCAGAATGAGATGAG | 273 |
MMP14 | NM 004995 | FWD: AGTCTCCCAGAGGGTCATTCA REV: GGTCCCATGGCGTCTGAAG | 320 |
SNAI 2 | NM 003068 | FWD: CTTTTTCTTGCCCTCACTGC REV: ACAGCAGCCAGATTCCTCAT | 161 |
t-ERG | FJ423744 | FWD: TMPRSS2_E1-TAGGCGCGAGCTAAGCAG REV: EGR_E4-GTCCATAGTCGCTGGAGGAG | 184 |
Vimentin | NM 003380 | FWD: GAGAACTTTGCCGTTGAAGC REV: TCCAGCAGCTTCCTGTAGGT | 170 |
ZEB 1 | NM 030751 | FWD: TGCACTGAGTGTGGAAAAGC REV: TGGTGATGCTGAAAGAGACG | 237 |