Fig. 7

L-PRP induced activation of NF-κB in cells in vitro. Western blotting was performed to analyze expression of NF-κB p65 in the nucleus of HBMSCs (a) and EaHy926 (b); RT-PCR was conducted to detect mRNA expression of COX-2 and iNOS of HBMSCs (c) and EaHy926 (d); ELISA and Griess reaction were performed to determine PGE2 and NO production, respectively, of HBMSCs (e) and EaHy926 (f). Bars represent the means and standard deviation (n = 5); * indicates the statistically significant difference between PRPs and FBS (P < 0.05); ^# indicates the statistically significant difference between P-PRP and L-PRP (P < 0.05)