Fig. 3

Lentivirus-mediated S100A13 knockdown inhibited thyroid cancer cell proliferation and invasion. a Growth curves of cells in each group. MTT analysis showed that lentivirus-mediated S100A13 knockdown significantly inhibited thyroid cancer TPC1 cell proliferation (n = 3). *P < 0.05. b Colony formation assay showed that lentivirus-mediated S100A13 knockdown significantly inhibited thyroid cancer TPC1 cell colony formation (n = 3, **P < 0.01). c Transwell invasion assay showed that lentivirus-mediated S100A13 knockdown significantly inhibited thyroid cancer SW579 cell invasion. Cells were transiently transfected with lentivirus-mediated shRNA plasmids and plated on the top of the transwells. Twenty-four hours after plating, cells invaded through the pores were counted. Values are expressed as mean ± SD of three independent experiments, *P < 0.05. d Scratch-wound assay showed that lentivirus-mediated S100A13 knockdown significantly inhibited thyroid cancer SW579 cell migration. e Western blot assay showed that lentivirus-mediated S100A13 knockdown inhibited expression of HMGA1 and Snail, promoted expression of E-cadherin in SW579 cells