Fig. 2

Ex vivo and in vitro stimulated (IVS) frequencies of CMV specific CD8⁺ T cells in AAD patients and controls. Immudex CMV dextramers were used to stain PBMC directly (ex vivo), or after the cells were stimulated with HLA-restricted CMV peptide (1 µg/mL) and expanded for 13 days (in vitro stimulated), for HLA-A2 or HLA-B8 CMV specific CD8⁺ T cells. All samples were analyzed on an Accuri C6 flow cytometer. Results are expressed as percentage of CD8⁺ T cells staining positive for CMV dextramers in a patients and controls. The same data are also shown grouped into HLA-B8 (b) and HLA-A2 (c) positive patients (b n = ex v. 9, IVS 6, c n = ex v. 6, IVS 4) and controls (b n = ex v. 10, IVS 9, c n = ex v. 6, IVS 4). Non-parametric Mann–Whitney U test was used to test for statistical differences between patients and controls, but none were found. The bars display the mean for the whole group