Fig. 5
From: Combination of double negative T cells and anti-thymocyte serum reverses type 1 diabetes in NOD mice
The accumulation of DN T cells in the pancreas-associated LN and the spleen as opposed to the MLN 6 months after combined ATS and DN T cell treatment. The spleen, pancreatic draining LN and mesenteric LN were harvested from different NOD mice and single cell suspensions were prepared. The percentage of CD4+, CD8+ and DN T cells in CD3+TCR-β+ T cells were examined by flow cytometry