Fig. 1
From: Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma
Exosomes (EXOs) purification and miR-222 expression analysis in melanoma. a Flow chart outlining the main steps of exosome isolation from cell culture supernatants. EXOs were purified from 24 h cell culture media by ultracentrifugation (UC) (left panel) or Exoquick-TC (EQ) (right panel) methods. miR-222 levels were compared by qRT-PCR in b stabilized melanoma cell lines and corresponding EXOs either UC (EXO UC) or EQ (EXO EQ) purified and c in early passages melanoma cell lines and corresponding EXOs. Me1007 primary melanoma cell line was used as an internal control to compare the two groups. Columns, mean ± SD of at least three independent experiments