Fig. 5
From: C14orf166 is a high-risk biomarker for bladder cancer and promotes bladder cancer cell proliferation
C14orf166 regulated the key proteins that regulate G1/S transition. a Cell cycle assay of the effect of C14orf166 knockdown on cell cycle progression. b Quantitative RT-PCR determination of Cyclin D1, P21, and P27 expression after C14orf166 knockdown in RT4 cells. Transcription levels were normalized to GAPDH expression. c Western blot determination of Cyclin D1, p21, p27, Rb, and p-Rb expression in RT4 cells. α-Tubulin was used as the loading control. d Quantitative RT-PCR determination of Cyclin D1, P21 and P27 expression following C14orf166 knockdown in T24 cells. Transcription levels were normalized to GAPDH expression. e Western blot determination of Cyclin D1, p21, p27, Rb, and p-Rb expression in T24 cells. α-Tubulin was used as the loading control. *P < 0.05